es2 ovarian cancer cell line atcc crl 1978 human  (ATCC)

Journal: International journal of oncology

Article Title: miR-28-5p promotes the development and progression of ovarian cancer through inhibition of N4BP1.

doi: 10.3892/ijo.2017.3915

Figure Lengend Snippet: Figure 2. miR‑28‑5p promotes the progression of cell cycle and proliferation and inhibits apoptosis in ovarian cancer cells. (A) The ability of cell proliferation was detected by clonal colony forming experiment in ES2 and SKOV3 transfected with miR‑28‑5p mimic, inhibitor or negative control (NC) respectively (**P<0.01). (B) MTT assay was performed to determine cell proliferation ability in ES2 and SKOV3 transfected with miR‑28‑5p mimic, inhibitor or NC respectively. The values of expression level are represented by the mean percent of means ± SD (n=3, **P<0.01). (C) The cell cycle distribution was detected by flow cytometry. (D) Ovarian cancer cells were transfected with miR‑28‑5p mimic, inhibitor or NC and cell apoptosis was detected by Annexin Ⅴ-FITC/PI staining (***P<0.001).

Article Snippet: Human epithelial ovarian cancer cell lines ES2 were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and SKOV3 cells were purchased from College of Life Science, Hunan Normal University, China.

Techniques: Transfection, Negative Control, MTT Assay, Expressing, Flow Cytometry, Staining

Journal: International journal of oncology

Article Title: miR-28-5p promotes the development and progression of ovarian cancer through inhibition of N4BP1.

doi: 10.3892/ijo.2017.3915

Figure Lengend Snippet: Figure 3. miR‑28‑5p expression accelerates migration and invasion ability of ovarian cancer cells. (A) The migration ability of ES2 and SKOV3 cells trans- fected with miR‑28‑5p mimic, inhibitor or NC were measured by wound healing assay at 0, 24 and 48 h respectively. (B) The migration assays of ES2 and SKOV3 cells transfected with miR‑28‑5p mimic, inhibitor or NC respectively were performed. Magnification, x200; scale bars, 10 µm (**P<0.01). (C) The invasion assays of ES2 and SKOV3 cells transfected with miR‑28‑5p mimic, inhibitor or NC respectively were performed. Magnification, x200; scale bars, 10 µm (**P<0.01).

Article Snippet: Human epithelial ovarian cancer cell lines ES2 were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and SKOV3 cells were purchased from College of Life Science, Hunan Normal University, China.

Techniques: Expressing, Migration, Wound Healing Assay, Transfection

Journal: International journal of oncology

Article Title: miR-28-5p promotes the development and progression of ovarian cancer through inhibition of N4BP1.

doi: 10.3892/ijo.2017.3915

Figure Lengend Snippet: Figure 4. miR‑28‑5p regulates F-actin, E-cadherin, and vimentin expression. (A) Western blot analysis detected the expression level of F-actin in ES2 and SKOV3 cells transfected with miR‑28‑5p mimic, inhibitor or NC. GAPDH was used as a protein-loading control. (B) The expression levels of E-cadherin and vimentin were measured by western blot analysis in ES2 and SKOV3 cells transfected with miR‑28‑5p mimic, inhibitor or NC.

Article Snippet: Human epithelial ovarian cancer cell lines ES2 were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and SKOV3 cells were purchased from College of Life Science, Hunan Normal University, China.

Techniques: Expressing, Western Blot, Transfection, Control

Journal: International journal of oncology

Article Title: miR-28-5p promotes the development and progression of ovarian cancer through inhibition of N4BP1.

doi: 10.3892/ijo.2017.3915

Figure Lengend Snippet: Figure 5. miR‑28‑5p downregulates N4BP1 expression in human ovarian cancer. (A) Representative images of N4BP1 immunohistochemical staining in ovarian cancer tissues and adjacent ovarian tissues, original magnification, x400. (B) miR‑28‑5p inhibited the mRNA expression level of N4BP1 in ES2 cells. The mRNA expression level of N4BP1 was detected by qRT-PCR in ES2 and SKOV3 cells transfected with miR‑28‑5p mimic, inhibitor or NC. (C) The expression level of N4BP1 was detected by western blot analysis in ES2 and SKOV3 cells transfected with miR‑28‑5p mimic, inhibitor or NC.

Article Snippet: Human epithelial ovarian cancer cell lines ES2 were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and SKOV3 cells were purchased from College of Life Science, Hunan Normal University, China.

Techniques: Expressing, Immunohistochemical staining, Staining, Quantitative RT-PCR, Transfection, Western Blot